Urine influences growth and virulence gene expressions in Uropathogenic E. coli: a comparison with nutrient limited medium

Objective. The interactions between environmental factors and microbial biological process are well known. Urine has been selected to provide host conditions probably affecting bacterial growth gene expressions. aim of this study was detect the modulations expressions [sfa/foc, cnf1, usp aer] UPEC in urine by comparing results with serum supplemented Standart American Petroleum Institute medium which is defined as host-like medium. Methods. UPECs were incubated 37°C alterations detected measuring changes absorbance at 600 nm four-,six- 24 hours periods. Gene expression levels analysed quantitative polymerase chain reaction (qPCR). Statistical analysis fold values growths calculated using one-way ANOVA Tukey's post hoc test. Results. increase found be statistically significant (p<0.0001). aer sfa/foc (p<0.001); whereas cnf1 genes not altered (p>0.05). Conclusion. According our results, an environment vivo affected both UPEC.